A continuous spectrophotometric method for the determination of leucine aminopeptidase.

A continuous method for the determination of leucine aminopeptidase in human serum with L-leucinamide as substrate.

A continuous procedure for the determination of leucine aminopeptidase is described. L-leucinamide is used as substrate and the liberated ammonia is determined with the glutamate dehydrogenase reaction. The enzyme is Mn2+-activated and 30 mumol/l MnCl2 is necessary for an optimal activity measurement. Influence of buffer type, buffer concentration and pH are reported together with the apparent ...

Solvent extraction, spectrophotometric determination of copper (II) from environmental samples using o-methylphenyl thiourea as a novel reagent

A simple and rapid method has been developed for solvent extraction and spectrophotometric determination of copper (II) using o-methylphenyl thiourea (OMPT) as a sensitive reagent. The basis of proposed method is formation of copper (II)-OMPT complex. Copper (II) was extracted with 0.020 mol L-1 OMPT in chloroform from aqueous solution in 0.075 mol L-1 potassium iodate. The absorbance of comple...

A simple spectrophotometric method for the determination of blood sugar by phenylhydrazine Hydrochlorlde

SPECTROPHOTOMETRIC REACTION RATE METHOD FOR THE DETERMINATION OF SLWR BASED ON ITS CATALYTIC EFFEICT ON THE OXIDATION OF GLLOCYANW BY PER0XYDISULPTE

A sensitive and selective procedure was developed for the spectrophotometric determination of silver by its catalytic effect on the oxidation of gallocyanine by peroxydisulphate in acidic media at 540 nm. Silver can be determined in the range of 0.001-0.500 µg/ml with a limit of detection of 0.0005 µg/ml, by a fixed-time method of 4.0 min from initiation of the reaction, The relative standa...

Kinetic Spectrophotometric Method for Determination of S032- Using Methylen blue

The present work reports a kinetic spectrophotometric method for the determination of traceamounts of sulphite that is based on inhibitory effect of sulphite on the oxidation of Methyleneblue (MB) in acidic media. The reaction is followed spectrophotometrically by monitoring thedecrease in absorbance of MB at 664 nm with a fixed time of 0.5-4.5 minutes. It was found thatsulphite exert a strong ...